pore size transwell insert  (Fisher Scientific)

Journal: APL Bioengineering

Article Title: Regulation of airway remodeling in asthma: The synergistic effects of nanotechnology and Hippo pathway activation

doi: 10.1063/5.0251258

Figure Lengend Snippet: In vitro mechanistic studies of CA-NPs-Quercetin on HASMCs. (a) CCK-8 assay measuring the proliferation rates of cells in each group at 12, 24, 36, 48, 60, and 72 h. (b) ECIS system monitoring real-time cell proliferation at 3, 6, 9, 12, and 15 h. (c) Flow cytometry analysis of apoptosis rates in each group. (d) Wound healing assay assessing the migration ability of cells in each group with images captured at 0, 24, and 48 h. (e) Transwell invasion assay evaluating the invasive capacity of cells in each group. * denotes significant differences between groups, *** p < 0.001.

Article Snippet: An 8 μm pore-sized Transwell insert (Corning, USA) was placed into a 24-well plate.

Techniques: In Vitro, CCK-8 Assay, Flow Cytometry, Wound Healing Assay, Migration, Transwell Invasion Assay

Journal: APL Bioengineering

Article Title: Regulation of airway remodeling in asthma: The synergistic effects of nanotechnology and Hippo pathway activation

doi: 10.1063/5.0251258

Figure Lengend Snippet: Effects of shMst1 and shLats1 on CA-NPs-Quercetin-induced proliferation and migration of HASMCs. (a) Immunofluorescence staining showing the localization of YAP and TAZ in cells. (b) Expression levels of YAP, TAZ, p-YAP, and p-TAZ in the cytoplasm and nucleus. (c) Wound healing assay assessing the migration ability of cells in each group, with images captured at 0, 24, and 48 h. (d) Transwell invasion assay evaluating the invasive capacity of cells in each group. (e) CCK-8 assay measuring the proliferation rates of cells in each group at 12, 24, 36, 48, 60, and 72 h. * denotes significant differences between groups, ** p < 0.01, *** p < 0.001.

Article Snippet: An 8 μm pore-sized Transwell insert (Corning, USA) was placed into a 24-well plate.

Techniques: Migration, Immunofluorescence, Staining, Expressing, Wound Healing Assay, Transwell Invasion Assay, CCK-8 Assay

Journal: APL Bioengineering

Article Title: Regulation of airway remodeling in asthma: The synergistic effects of nanotechnology and Hippo pathway activation

doi: 10.1063/5.0251258

Figure Lengend Snippet: Activation of the Hippo signaling pathway by CA-NPs-Quercetin in vivo . (a) Schematic representation of the primary cell isolation and experimental protocol in mice. (b)–(d) Western blot analysis showing the phosphorylation levels of YAP and TAZ in primary airway smooth muscle cells from mice. (e) Transwell migration assay evaluating the migration capacity of primary airway smooth muscle cells in each group of mice. (f) Wound healing assay assessing the migration ability of primary airway smooth muscle cells in mice. * denotes significant differences between groups, ns indicates p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: An 8 μm pore-sized Transwell insert (Corning, USA) was placed into a 24-well plate.

Techniques: Activation Assay, In Vivo, Cell Isolation, Western Blot, Phospho-proteomics, Transwell Migration Assay, Migration, Wound Healing Assay

Journal: European Journal of Medical Research

Article Title: EMP3 is upregulated upon epithelial–mesenchymal transition and contributes to EGFR–tyrosine kinase inhibitor resistance in lung adenocarcinoma

doi: 10.1186/s40001-025-02894-9

Figure Lengend Snippet: EMP3 overexpression promotes TKI resistance and stemness in LUAD cells. An EMP3 overexpression plasmid, or an empty vector, was administered to parental HCC827 or H1975 cells. a , b mRNA and protein levels of EMP3 in cells after transfection determined using qPCR and WB analyses. c , d Viability of HCC827 or H1975 cells under different concentrations of osimertinib or erlotinib treatment determined using CCK-8 assays. e Phosphorylation of EGFR in cells under osimertinib or erlotinib treatment determined using WB analysis. f Proliferation of cells determined using CCK-8 assays. g Colony formation ability of cells determined using colony formation assays. h Migration of cells determined using transwell assays. i Population of CD133. + cells determined using flow cytometry. j Sphere formation capacity of cells determined using sphere formation ability. Six independent experiments were conducted. ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Briefly, 1 × 10 5 transfected cells were resuspended in serum-free medium and placed in the upper chambers of transwell inserts (8 μm pore size, Corning).

Techniques: Over Expression, Plasmid Preparation, Transfection, CCK-8 Assay, Phospho-proteomics, Migration, Flow Cytometry

Journal: European Journal of Medical Research

Article Title: EMP3 is upregulated upon epithelial–mesenchymal transition and contributes to EGFR–tyrosine kinase inhibitor resistance in lung adenocarcinoma

doi: 10.1186/s40001-025-02894-9

Figure Lengend Snippet: Inhibition of the TGF signaling enhances TKI sensitivity in EMP3 overexpressing cells. EMP3-overexpressing HCC827 and H1975 cells with the TGF signaling inhibitor LY2109761 or DMSO. a , b Viability of HCC827 or H1975 cells under different concentrations of osimertinib or erlotinib treatment determined using CCK-8 assays. c Phosphorylation of EGFR in cells determined using WB analysis. d Proliferation of cells determined using CCK-8 assays. e Colony formation ability of cells determined using CCK-8 assays. f Migration of cells determined using transwell assays. g Population of CD133. + cells determined using flow cytometry. Six independent experiments were conducted. ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Briefly, 1 × 10 5 transfected cells were resuspended in serum-free medium and placed in the upper chambers of transwell inserts (8 μm pore size, Corning).

Techniques: Inhibition, CCK-8 Assay, Phospho-proteomics, Migration, Flow Cytometry

Journal: bioRxiv

Article Title: Developmental Immunotoxicity of Low-dose Inorganic Arsenic Reprograms Macrophages Inducing Tumor-promoting Phenotypes

doi: 10.1101/2025.07.14.663593

Figure Lengend Snippet: Transwell migration assay was used to measure migratory capacity of exposed M0 female and male BMDMs toward LLC1 and KLN205 lung cancer cell conditioned media. n=3-5 biological replicates. Plots show mean ± SD of percent migration measured with crystal violet staining. **p<0.01. iAs= inorganic arsenic; BMDMs= bone marrow-derived macrophages.

Article Snippet: To evaluate the impact of arsenic-exposed macrophages on cancer cell proliferation, indirect coculture experiments were conducted using 0.4 μm pore size transwell inserts (Corning, Corning, NY).

Techniques: Transwell Migration Assay, Migration, Staining, Derivative Assay